[institut] Seminar Wolfgang Fritzsche

[Brana Jelenkovic]

                    SEMINAR 

Sreda 17 april, Biblioteka Dragan Popovic, 13 h. 

    BIOANALYTICS USING PLASMONIC NANOSTRUCTURES

              Wolfgang Fritzsche

_Leibniz Institute of Photonic Technology (IPHT), A.-Einstein-Str. 9,_
_                  Jena, Germany_ 
fritzsche at ipht-jena.de

ABSTRACT: Novel requirements for bioanalytical methods emerge due to
trends such as personalized medicine or pathogen monitoring in
environment and food. Here, innovative tools for diagnostics are needed,
to be used outside of dedicated laboratories and with less qualified
personnel, at minimal costs. Plasmonic nanostructures promise to provide
sensing capabilities with the potential for ultrasensitive and robust
assays in a high parallelization, and without the need for marker. Upon
binding of molecules, the localized surface plasmon resonance (LSPR) of
these structure is changed, and can be used as sensoric readout [1].
This is possible even on a single nanostructure level, using optical
darkfield detection introduced more than 100 years ago [2], as
demonstrated for DNA detection [3]. In contrast to SPR, LSPR senses only
in a very thin layer (on the scale of the particle diameter), resulting
in an efficient background suppression [4].
In order to multiplex this approach, an imaging spectrometer based on a
Michelson interferometer has been developed, able to readout a whole
array of sensors in one step [5]. On the sensor side, microarrays of
gold nanoparticle spots were fabrictaed using spotting of
pre-synthesized gold nanoparticles [6].  Such chemically synthesized
particles allow for a cost-efficient generation of highly crystalline
particles as nanosensors; by using microfluidic approaches, a high
quality and reproducibility can be achieved [7]. The functionalization
of the various particle spots is realized by spotting thiolized DNA onto
each spot separately. Using this approach, a multiplex DNA-based
detection of fungal pathogens involved in sepsis was possible [8]. 
Besides sensing, individual plasmonic nanostructures can be also used to
optically manipulate biomolecular structures such as DNA. Attached
particles can be used for local destruction [9] or cutting as well as
coupling of energy into (and guiding along) the molecular structure upon
laser irradiation [10]. The resonance wavelength of these particles can
not only manipulated by their inherent properties (material, geometry)
or their surrounding, but also by coupling with adjacent metal films due
to interferometric effects [11] or gap modes. This effects is also
usable for a novel sensing approach by inserting a flow-through channel
between particle and the metal film mirror, analyte particle moving
through the channel will change the refractive index in the space
between particle and mirror and thereby change the LSP resonance
observed.

[1]    A. Csaki, T. Schneider, J. Wirth, N. Jahr, A. Steinbrück, O.
Stranik, F. Garwe, R. Müller and W. Fritzsche, Philosophical
Transactions A 369, 3483-3496 (2011).
[2]    T. Mappes, N. Jahr, A. Csaki, N. Vogler, J. Popp, W. Fritzsche.
Angew Chem Int Ed 51, 11208-11212 (2012)
[3]    T. Schneider, N. Jahr, A. Csaki, O. Stranik and W. Fritzsche, J
Nanopart Res 15, 1531 (2013)
[4]    J. Jatschka, A. Dathe, A. Csaki, W. Fritzsche, O. Stranik.
Sensing and BioSensing Research 7, 62-70 (2016)
[5]    D. Zopf, J. Jatschka, A. Dathe, N. Jahr, W. Fritzsche, O.
Stranik. Biosensors and Bioelectronics 81, 287-293 (2016)
[6]    A. Pittner, S. Wendt, D. Zopf, A. Dathe, N. Grosse, A. Csaki, W.
Fritzsche, O. Stranik. Analytical and Bioanalytical Chemistry (2019)
accepted
[7]     Thiele M, Soh J Z E, Knauer A, Malsch D, Stranik O, Müller R,
Csáki A, Henkel T, Köhler J M and Fritzsche W 2016 Chemical Engineering
Journal 288 432-40
[8]    D. Zopf, A. Pittner, A. Dathe, N. Grosse, K. Arstila, J. Toppari,
W. Schott, D. Dontsov, G. Uhlrich, W. Fritzsche, O. Stranik. ACS Sensors
(2019) in revision 
[9]    A. Csaki, F. Garwe, A. Steinbrück, G. Maubach, G. Festag, A.
Weise, I. Riemann, K. König and W. Fritzsche, Nano Letters 7 (2),
247-253 (2007).
[10]    J. Wirth, F. Garwe, G. Haehnel, A. Csaki, N. Jahr, O. Stranik,
W. Paa and W. Fritzsche, Nano Letters 11 (4), 1505-1511 (2011).;  J.
Toppari, J. Wirth, F. Garwe, O. Stranik, A. Csaki, J. Bergmann, W. Paa,
W. Fritzsche, ACS Nano 7, 1291-1298  (2013); J. Wirth, F. Garwe, J.
Bergmann, W. Paa, A. Csaki, O. Stranik, W. Fritzsche. Nano Letters 14,
570-577 (2014)
[11]    J. Wirth, F. Garwe, R. Mayer, A. Csaki, O. Stranik, W.
Fritzsche: Nano Letters 14, 3809-3816 (2014)
-- 
Brana Jelenkovic
Photonics Center 
Institute of Physics
University of Belgrade
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