[institut] Photonics Center Seminar, Tuesday, 28. 04. 2026. at 12h

[Mihailo Rabasovic]

Dear colleagues,

You are kindly invited to the Photonics center seminar, which will be
held on Tuesday, 28. 04. 2026. at 12h, in the "Dragan Popović" hall. The
Laboratory for Biophysics will be presented at the seminar. Aleksandar
Krmpot will tell us something about the lab, and Marta Bukumira will
give a seminar entitled:

3 in 1: imaging, surgery, electrophysiology

The seminar abstract is below in the e-mail.

Best regards,
Mihailo Rabasović

Abstract
Understanding cellular function requires approaches that can link
structure, manipulation and electrophysiological response within a
single experiment. This is particularly challenging in systems such as
filamentous fungi, where rigid cell walls limit access for functional
measurements. 

In this seminar, a multimodal platform will be presented that integrates
nonlinear optical imaging, femtosecond laser cell surgery and patch
clamp electrophysiology. The system enables precise targeting of regions
of interest and controlled removal of the cell wall, allowing direct
access to the protoplast. 

Using _Phycomyces blakesleeanus_ as a model system, the approach
addresses key experimental challenges, eliminating sample transfer and
enabling measurements under near-native conditions. The instrument
provides a way to directly correlate cellular structure and function,
and is not limited to fungal systems, but can be applied to a wide range
of biological samples requiring advanced imaging, laser-based treatment
and precise mechanical manipulation. Due to its broad applicability, it
has already attracted interest from both national and international
research groups. 

The presented work was carried out within the BioPhysFUN project, funded
by the Science Fund of the Republic of Serbia, and represents a central
part of the author's doctoral research.
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